Lindelofiastylosa (Boraginaceae) is reported for the first time from Iran. The specimen of this species has been collected from Kuh-e Karkasi (Dibaj, N Damghan, Semnan province) during ongoing floristic studies and research journeys to the southern slopes of the Alborz Mountains. Molecular evidence obtained from DNA extraction and Internal Transcribed Spacer (ITS) sequence analysis confirm this new record. Description, photographs of living plantand some associated species areprovided.