Abstract
Background & Aims: Neurogenesis in the adult mammal brain occurs throughout life. Adult neurogenesis has been clearly
demonstrated in the sub granular zone (SGZ) of the dentate gyrus (DG) in the hippocampus. Pheromones that plays an essential role
in the development of the central nervous system. The male pheromones are involved in regulating neurogenesis in both the olfactory
bulb and hippocampus, which may be important for female reproductive success. The golgi silver impregnation method is a powerful
method still routinely used for studying neuronal morphology. The aim of the present study was to investigate the effects of pheromones
on the neuronal morphology in the mice hippocampus.
Materials and Methods: Thirty adult NMRI mice 6-8 weeks were used in this study. Adult female animals were divided into four
groups. These intact and bilaterally ovariectomized mice and males were placed with and without wire mesh in cage. The animals were
sacrificed by cervical dislocation and brain was sectioned (50 μm) and stained with golgi method and observed by microscope.
Results: Neuronal arborization in pheromones group has different with and without pheromones groups. The morphology features of
the dentate gyrus neurons of the hippocampus were varied in the exposure to the pheromones as compared to other group.
Conclusion: This study report that the pheromones of males stimulate neurogenesis and neuronal morphology in the hippocampus of
female mice.
Key words: Golgi method, Neurogenesis, Hippocampus, Pheromones