Abstract
Introduction: Rosemary leaf extract, as a medicinal plant, can stimulate
stem cells proliferation. Human adipose stem cells (HASCs) are readily
available and less harmful accessibility with high therapeutic
application.
Methods: In this study, fat tissue was isolated from Caesarean women
at Damgan Hospital. After mechanical and enzymatic digestion,
extracted cells were cultured in α–MEM containing 10% FBS. Stemness
of HASCs was confirmed by flow cytometry and their differentiation
potential. The survival rate, proliferation and neurotrophins genes
expression of cells treated with 30, 40, 50, 60 and 70 µg/ml
concentrations of Rosemary extract containing 40% of carnosic acid for
24 and 48h were evaluated using hoemocytometry, MTT and PCR
assays.
Results: Cultured HASCs showed spindle and fibroblastic shapes under
microscopic study. They expressed positive response to CD44, CD73,
CD90, CD105 and negative response to CD34 and CD45markers and
differentiated into adipocytes and osteocytes. The proliferation rate and
survival of cells treated with Rosemary at a concentration of 50 μg/ml
for 48 h were increased significantly as compared to cultured cells in
medium without Rosemary, containing serum.
Conclusion: Rosemary extract could be used as a serum supplement to
prepare more suitable cells for transplantation.

Keywords: Human Adipose Stem
Cells, Rosemary Extract, Proliferation,
Survival.